How to know my T cells are activated

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Is there a visible sign that my T cells are activated after incubating with Aim-Tconv HMP?

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Yes, there are visible signs that indicate T cell activation following incubation with Aim-Tconv HMP. Activated T cells typically exhibit the following characteristics:

  1. Morphology Changes: Activated T cells are larger in size and have an irregular shape compared to resting T cells, which are smaller and round.
  2. Surface Markers: You can identify activated T cells through specific surface markers using FACS (Fluorescence-Activated Cell Sorting). For information on which markers correspond to specific T cell types, please refer to the protocol.

Protocol:
https://uploads.strikinglycdn.com/files/26721146-321b-4cae-a37f-eb88da95933a/AimTconv%20human%20protocol-1.1.3-bilingual.pdf?t=1741050466&id=4247533